Genomics and Genome Technologies

The Genomics Core Facility at QBRI actively supports scientific research by providing advanced research technologies and methods to the research community at HBKU and QBRI, and their collaborators from academia and industries to address the complex biomedical problems of QBRI’s research interests in the field of cancer biology, metabolic disorders and neurological diseases. The genomics core laboratory is equipped with various state-of-the-art, high-throughput technological platforms to sequence the nucleic acids by next-generation sequencing (NGS) as well by capillary sequencing methods; high-throughput genotyping studies by Illumina iScan system; and to study the expression of genes by NGS technologies, single cell platforms and targeted quantitative and digital PCR methods.

Genomics core laboratory operation is aligned to support the vision and mission of QBRI based on the following fundamentals;

• Assisting the researchers with design of an experiment, providing intellectual support and novel method development for genomics experiments;
• Advanced training in genome technologies for the graduate students and researchers;
• Interface with other core technological platforms to facilitate integrated biology research;
• Collaborative research.

Facilities and Capabilities

The facilities and instruments that are available at QBRI Genomics Core Facility are classified broadly under the following categories

• Genome Sequencing
  • Illumina HiSeq 4000 and 2500 (high throughput sequencing of genomes and transcriptomes)
  • Illumina MiSeq (for smaller genome. amplicon sequencing and targeted Sequencing)
• Large Scale Genotyping Analysis
  • Illumina iScan systems (Illumina Bead Array technology for high-throughput SNP genotype analysis)
  • AB 3730xl 96 capillaries and 3100xl 16 capillaries Genetic Analyzers (for standard targeted sequencing and fragment analysis)
• Targeted Gene Expression Analysis
  • QuantStudio 12K Plus for qPCR and Digital PCR and Gene Expression Panels studies
  • NanoString Technologies (targeted gene expression for up to 800 genes)
  • AB 7900 Real-Time PCR System
• Single Cell Analysis
  • Fluidigm C1 Single-Cell Auto Prep & BioMark HD Systems
  • Fluidigm Access Array Sample Enrichment (efficient library preparation)
  • BIO-RAD ddSEQ System

Core Laboratory Services

• Advice on experimental design and sample preparation
• Quality control of nucleic acid materials by LabChip, QuBit and Bioanalyzer
• Different types of sequencing library preparation and data generation by high-throughput sequencing methods
• Quantitative PCR for differential gene expression analysis
• Training and education in genome technologies and data analysis
• Helping researchers with the generation of preliminary data for grant applications
• Data management and storage

Sample Submission Forms and Pricing

Please contact Dr. Kumaran Mande, Core Manager for Genomics Facilities at kmande@hbku.edu.qa

3730xl DNA Analyzer (Life Technologies)	iScan System (Illumina)	SOLiD 5500xL (ABI)
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IonTorrent/PGM (Life Technologies)	MiSeq (Illumina)	HiSeq 2500 (Illumina)
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Thunderstorm High-Throughput Sequencing System
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